Hoxb8-fl
WebTo test if Cas9-Hoxb8 cell-derived DCs rely on the same mechanisms and thus can be exploited as a tool to study DC migration, we used lentiviral-based CRISPR/Cas9 … Web13 feb. 2024 · Hoxb8‐FL cells, homozygous for membrane GFP (mG), were infected with lentiviruses coding for puromycin resistance, Cas9 and a guide RNA (gRNA) 13 directed …
Hoxb8-fl
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WebHoxb8-FL cells are conditionally immortalized HSPCs that resemble MPPs by maintaining both lymphoid and myeloid potential (Redecke et al., 2013). We find that the EBF1 … WebTreatment of ERHBD-Hoxb8-expressing progenitors with estrogen results in Hoxb8 activation, which blocks cell differentiation and allows for expansion of homogenous progenitor cell populations in the presence of FLT3L. These cells, referred to as Hoxb8-FL cells, retain lineage potential for lymphocyte and myeloid lineages, including the DC …
BM– and Hoxb8-FL–derived DCs and macrophages were generated by cultivating unfractionated BM cells or Hoxb8-FL cells for 6 d in standard growth medium (RPMI 1640 (Invitrogen) for DCs or DMEM (Invitrogen) for macrophages, supplemented with 10% (v/v) FCS (Hyclone), 50 mM 2-mercaptoethanol and … Meer weergeven Antibodies used were against B220 (RA3-6B2, dilution 1:200), CD4 (RM4-5, dilution 1:200), CD45.1 (A20, dilution 1:200), MHC class II … Meer weergeven BM cells were collected by flushing femurs of 4–8-week-old female C57BL/6J or B6.SJL mice with 10 ml RP-10 (RPMI 1640 … Meer weergeven The plasmids MSCV-ERHBD-Hoxb8 and the ecotropic packaging vector pCL-Eco (Imgenex) were both transfected into HEK293T … Meer weergeven Hoxb8-FL cells and BM cells were differentiated in the presence of M-CSF contained in L-cell conditioned medium for 7 d, followed by incubation with FITC-labeled IgG-coated beads (1:100, Cayman Chemical, … Meer weergeven Web5 jun. 2024 · The HoxB8-FL cell line was recently reported to have both myeloid and lymphoid potential . We therefore also generated expression profiles for 107 HoxB8-FL single cells, which, when projected onto the diffusion plot, confirmed that the HoxB8-FL expression state resembles that of primary bone marrow cells from the LMPP trajectory.
WebHoxb8-FL cells were transduced with MLL-ENL or empty virus to generate ME-Parental and Parental cells, respectively. When cultured in methylcellulose without Flt3L and estradiol (causing... Web1 feb. 2024 · Hoxb8 conditionally immortalised macrophage lines model inflammatory monocytic cells with important similarity to dendritic cells. We have examined the …
Web26 okt. 2024 · Hoxb8-FL cell line is a model system of lympho-myeloid progenitors, which self-renews in vitro and is amenable to genetic perturbations. To construct a functionally …
Web29 nov. 2024 · Serial monitoring of peripheral blood demonstrated that Hoxb8-FL derived cells had disappeared by 4 weeks, and did not reappear. However, at 6 months CEBPA … thermopolis wyoming area codeWeb13 feb. 2024 · Hoxb8-FL cells, homozygous for membrane GFP (mG), were infected with lentiviruses coding for puromycin resistance, Cas9 and a guide RNA (gRNA) 13 directed … toyworld moreeWebData are representative for at least five independent populations of Hoxb8–FL cells. ( b ) Three–dimensional PCA based on gene expression profiles of Hoxb8–FL cells (four … thermopolis wyoming 3 on 3Web29 nov. 2024 · Serial monitoring of peripheral blood demonstrated that Hoxb8-FL derived cells had disappeared by 4 weeks, and did not reappear. However, at 6 months CEBPA N321D-transduced cells could still be detected in bone marrow in contrast to EV-transduced cells but without any leukemic phenotype. toyworld morningtonWeb1 jan. 2024 · Flow cytometry and single-cell transcriptomic characterisation of cells at different time points revealed that the mutation stalls the multipotent HOXB8-FL cell line along the differentiation trajectory resulting in a self-renewing progenitor-like state exhibiting haematopoietic markers indicative of a plasmacytoid dendritic cell-like phenotype. thermopolis wyoming apartmentsWebHoxb8-FL cells have lost self-renewal capacity and potential to differentiate into megakaryocytes and erythrocytes but retain the potential to differentiate into myeloid and … thermopolis wyoming airportWebHere, I am describing the method to establish Hoxb8-FL cells from mouse BM, as well as procedures for DC generation and gene deletion using lentivirally delivered … thermopolis wyoming archaeopteryx glider