WebTherefore, this precise and sensitive LCAT assay will help clarify the role of this enzyme in the metabolism of HDLs, and can be used for diagnostic purposes in investigating liver function. We obtained five monoclonal antibodies that recognized different epitopes on LCAT and developed a sandwich-type ELISA. Webby fluorescent LCAT assay kit (Roar Biomedical, USA). In this method, the fluorescent labeled LCAT substrate is exposed to and incubated with human serum at 37º C for 4-8 hours.
Development of a novel fluorescent activity assay for lecithin
WebAssay kit useful for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in human plasma. LCAT mediates the formation of cholesteryl esters in … WebBackground Lecithin:cholesterol acyltransferase (LCAT) is a plasma enzyme that esterifies cholesterol. Recombinant human LCAT (rhLCAT) is now being developed as an enzyme … companion of the avatar
Lecithin Cholesterol Acyltransferase (LCAT) Activity Assay Kit ...
Web5. Determine the change in emission ratio of 470 nm/390 nm over time to compare LCAT activity among samples using an excitation wavelength of 340 nm. Related Product Roar LCFC-LCAT Activity Assay Kit (cat. #RB-LCFC), 100 assays: useful for measuring the acyltransferase activity of LCAT resulting in the formation of cholesteryl ester. WebAlthough the two assays differed in their absolute units of LCAT activity, there was a good correlation between the two test assays (r¼0.849, P<1.6 10 7,y¼0.1378x þ 1.106). The BODIPY-cholesterol assay had an intra-assay CVof 13.7%, which was superior to the intra-assay CV of 20.8% for the radioisotopic assay. The proteoliposome substrate WebLCAT assay: The rate of FC esterification in the serum was assessed by measuring LCAT activity with a slight modification of the method of Dieplinger and Kostner (12). Freshly collected duplicate serum samples (20 ,ll) were pipetted into two test tubes, which were then closed with glass stoppers. companion of the bath wikipedia