Plko.1 tet on

Author: lfuu

August undefined, 2024

WebTET-pLKO.1 PURO shGDF11 #1. Plasmid #83083. Purpose. Lentiviral shRNA vector for inducible knockdown of human GDF11 (cross reacts with mouse Gdf11) Depositor. Kevin … Web本发明公开了抑制PHB基因的物质与IR在胶质瘤治疗中的联合应用。本发明发现PHB基因的敲除或敲低联合放疗可以促进脑肿瘤细胞的死亡，抑制肿瘤的生长，并延长荷瘤小鼠的生存期，说明，PHB缺失能够增加GBM移植瘤对放疗的敏感性并改善其治疗效果，PHB可以作为治疗GBM患者的潜在靶点。本发明具有 ...

抑制PHB基因的物质与IR在胶质瘤治疗中的联合应用【掌桥专利】

WebApr 15, 2024 · Lung cancer is the most prevalent malignancy and the first leading cause of cancer deaths across the globe and accounting for approximately 18% of all cancer … WebTo do this we need to add 5'-AATTC and G-3' to the top oligo and 3'-G and CAGCT-5' to the bottom oligo making our final oligos 34bp each. We could leave off the 3’ G on each oligo … cyclone\\u0027s i0

pLKO-Tet-On User Manual revised - Addgene

WebDec 7, 2015 · Novartis pLKO - Tet - On Prepare transfection mixture of plasmid DNA and Fugene transfectionreagent (Roche) according to manufacturer’s instructions. A 1:3 ratio … WebFor more information, see the Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse transcription, and integration needed for the production of lentiviral particles. It’s capable of ... WebEfficient cleavage requires at least two copies of the BspMI recognition sequence. cyclist janez brajkovic

Selecting the optimal Tet‐On system for doxycycline‐inducible …

pLKO.1 puro Sequence and Map - SnapGene

WebPlasmid pLKO.1-TetON-Puro-HIF1a 0819 from Dr. William Kaelin's lab contains the insert shRNA against human HIF1a and is published in Cell. 2016 Jun 30;166(1):126-39. doi: … cyclone® v se 5cseba6u23i7Web(human phosphoglycerate kinase 1 promoter): 2912–3422 Tet-On® 3G (transactivator gene): 3441–4187 P SV40 (SV40 promoter): 4198–4527 PuroR (puromycin resistance gene): 4536–5135 WPRE (woodchuck hepatitis virus posttranscriptional regulatory element): 5149–5737 3' LTR (3’ long terminal repeat): 5945–6578 اندازه ابعاد پست اینستاگرام

"Web(pLKO-Tet-On) was generated to allow for the rapid generation of multiple stable cell lines with regulatable shRNA expression. We demonstrate the advantages and versatility of … " - Plko.1 tet on

Plko.1 tet on

WebThe pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has built a library of shRNAs directed against 15,000 human and 15,000 mouse genes. Addgene is … Web我用的是pLKO-Tet-On质粒shRNA，包病毒，包装质粒是pVSV-G，delta8.9，想请教一下这样的包装系统合理吗？哪位童鞋用过pVSV-G，delta8.9 包病毒，比例多少比较合适. 丁香园-核酸基因技术-2015-04-22 17:43:56

Did you know?

WebFeb 1, 2009 · Our data show that pLKO-Tet-On-mediated knockdown is tightly regulated by the inducer tetracycline and its derivative, doxycycline, in a concentration- and time … WebHi all, I have been trying to produce viral particles containing pLKO-tet-on plasmid in HEK cells. I am using Addgene's 3rd generation packaging system (pMDLg/pRRE, pRSV …

WebAfter transduction to HeLa cell using lentiviral vector which contain tet-pLKO-puro vector, I treated puromycin 0.5ug. I made puromycin cell media mixed with DMEM(10% FBS, … WebAddgene inc plko tet puro vector Plko Tet Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

WebSep 3, 2015 · The doxycycline (dox)-inducible Tet-On system is widely used to control gene expression in mammalian cells. This system is based on the bacterial Tet operon, which has been modified and improved for its function in eukaryotic cells. To identify the optimal system for different applications, we compared Tet-On variants in frequently … WebpSuper pSuper retro GFP-neo pSupeior puro pBabe puro pCMV-Gag-pol pCMV-VSV-G pCL Ampho pSuper retro puro pLVX-shRNA1 pLVX-shRNA2 pSicoR pSicoR pGK puro pLentilox 3.7 pLKO.1 puro PLKO.1 pLKO.3G pTet-PLKO-puro pPRIME-TET-GFP-FF3 pPRIME-TREX-GFP-FF3 pSIH1-H1-CoGFP pGIPZ empty pGIPZ control pTRIPZ empty pTRIPZ …

WebDec 18, 2024 · 1、四环素调控表达系统的基本原理. Tet调控表达系统通过诱导药物（如Tet）改变调控蛋白的构象，从而达到调控目标蛋白表达的目的。 Tet调控基因表达系统 …

WebSep 21, 2024 · Mouse KrasG12D/P53-/-(KP) lung tumor cells were transfected with pLKO.1-Tet on-shNpm1-3(N3), pLKO.1-Tet on-shNpm1-4(N4) or pLKO.1-Tet on-shNpm1-5 (N5) and then selected by using 2ug/ml puromycin, to establish the 3 stable cell lines. These 3 stable cell lines (N3, N4 or N5) were treated with 100ng/ml Doxycline and then the total … cyberslim s1 u3h驅動程式WebThe MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones. 1] [ 2] This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones. cyclocarya paliurus batalin iljinskajaWebSeven out of eight Tet-One clones show more than 1,000-fold induction of expression. HeLa cells were infected with LVX-TetOne-Puro-Luc lentivirus (M.O.I. = 1), and eight individual clones were selected and expanded according to the protocol. Each clone was analyzed for doxycycline-induced expression of luciferase. cyclone\\u0027s ijWeb质粒载体网zlzt.com专业提供Tet-pLKO-Puro-NAP1L3(mouse)-shRNA1,产品编号为zl-006712,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完美质粒众筹,质粒合成,质粒构建,质粒提取,质粒保藏! cyclobenzaprine drug bankWebFeb 28, 2024 · METHODS: First, we modified the Tet-pLKO-Puro vector to make it easy ("EZ") for molecular cloning (EZ-Tet-pLKO-Puro). Our primary modification was to shrink the stuffer region, which allows vector purification via polyethylene glycol precipitation thereby avoiding the need to purify DNA through agarose. In addition, we generated EZ-Tet … اندازه برگه a3 در فتوشاپWeb目的观察赖氨酸特异性去甲基化酶1(LSD1)、N-myc下游调节基因1(NDRG1)基因沉默对人卵巢癌SKOV3细胞迁移能力的影响，并探讨两者的作用关系。 ... 自美国Upstate公司；兔抗人α-tubulin单克隆抗体购自美国Bioworld Technology公司。慢病毒表达质粒pLKO-Tet-On、pHR′-CMV-8.2ΔVPR和pHR ... cyclone brazilian jiu-jitsuWebFeb 28, 2024 · Vector maps and PEG purification. a Basic vector maps (not to scale) for the original Tet-pLKO-Puro vector and our modified versions.b Agarose gel electrophoresis comparing DNA precipitation methods. 10 μg of EZ-Tet-pLKO vector DNA was co-digested with NheI + EcoRI. The digest was split into three 3 μg aliquots and precipitated with … cyclone\\u0027s nj